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α ncam 1  (R&D Systems)


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    R&D Systems α ncam 1
    α Ncam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+ncam+1/pmc11394063-56-87-88?v=R%26D+Systems
    Average 95 stars, based on 37 article reviews
    α ncam 1 - by Bioz Stars, 2026-07
    95/100 stars

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    Proteomic analysis of polysialylated proteins from NK-92 cells. A) Immunoblot analysis of NK-92 cell lysate samples treated with PBS or with active EndoN before isolation on immobilized EndoN DM . α-polySia and α-NCAM blots show lysate (L), flowthrough (F), wash (W), and boiled beads (B). B) Volcano plot of proteins identified in samples relative to those pretreated with active EndoN. Proteins with a significant difference are labelled. The known polysialylated proteins <t>NCAM1</t> and ST8Sia4 are highlighted ( n = 4 per group, assessed with student’s T-test).
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    Proteomic analysis of polysialylated proteins from NK-92 cells. A) Immunoblot analysis of NK-92 cell lysate samples treated with PBS or with active EndoN before isolation on immobilized EndoN DM . α-polySia and α-NCAM blots show lysate (L), flowthrough (F), wash (W), and boiled beads (B). B) Volcano plot of proteins identified in samples relative to those pretreated with active EndoN. Proteins with a significant difference are labelled. The known polysialylated proteins NCAM1 and ST8Sia4 are highlighted ( n = 4 per group, assessed with student’s T-test).

    Journal: Glycobiology

    Article Title: Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein

    doi: 10.1093/glycob/cwae026

    Figure Lengend Snippet: Proteomic analysis of polysialylated proteins from NK-92 cells. A) Immunoblot analysis of NK-92 cell lysate samples treated with PBS or with active EndoN before isolation on immobilized EndoN DM . α-polySia and α-NCAM blots show lysate (L), flowthrough (F), wash (W), and boiled beads (B). B) Volcano plot of proteins identified in samples relative to those pretreated with active EndoN. Proteins with a significant difference are labelled. The known polysialylated proteins NCAM1 and ST8Sia4 are highlighted ( n = 4 per group, assessed with student’s T-test).

    Article Snippet: The antibodies used in this manuscript consisted of α-polySia mAb 735 (BioAspect BA-Ab00240-2.0), α-CD56 (R&D systems, mAB2408), α-beta-tubulin (Abcam, ab6046), streptavidin-HRP (Biolegend, 405210), goat α-mouse IgG-HRP (R&D systems, HAF007), goat α-rabbit IgG-HRP (Abcam, ab6721), and α-QSOX2 (Abcam, ab191168 and ab121376).

    Techniques: Western Blot, Isolation